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mouse anti human cfh  (R&D Systems)


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    R&D Systems mouse anti human cfh
    Mouse Anti Human Cfh, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human cfh/product/R&D Systems
    Average 93 stars, based on 24 article reviews
    mouse anti human cfh - by Bioz Stars, 2026-06
    93/100 stars

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    FIGURE 2. Cd increases <t>CFH</t> expression and secretion in HRGECs. (A, C, and E) ELISA of CFH secretion from HRGECs (A), human renal podocytes (HRPs) (C), and HRMCs (E) treated with 4 mM Cd (n = 4). (B, D, and F) mRNA expression of CFH gene in HRGECs (B), HRPs (D), and HRMCs (F) treated with 4 mM Cd (n = 4). Note: the scales are different in the y-axes. (G) Immunoblots of CFH from culture media of HRGECs. Coomassie Blue staining of the gel was presented as the loading control (n = 4). (H) Immunoblots of CFH from cell lysates of <t>HRGECs.</t> <t>GAPDH</t> was presented as the loading control (n = 4). (I) Immunoflourescence of CFH on cultured HRGECs treated with 4 mM Cd for 24 h. Positive staining of CFH was shown by orange-red fluorescence (n = 4). The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.
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    FIGURE 2. Cd increases <t>CFH</t> expression and secretion in HRGECs. (A, C, and E) ELISA of CFH secretion from HRGECs (A), human renal podocytes (HRPs) (C), and HRMCs (E) treated with 4 mM Cd (n = 4). (B, D, and F) mRNA expression of CFH gene in HRGECs (B), HRPs (D), and HRMCs (F) treated with 4 mM Cd (n = 4). Note: the scales are different in the y-axes. (G) Immunoblots of CFH from culture media of HRGECs. Coomassie Blue staining of the gel was presented as the loading control (n = 4). (H) Immunoblots of CFH from cell lysates of <t>HRGECs.</t> <t>GAPDH</t> was presented as the loading control (n = 4). (I) Immunoflourescence of CFH on cultured HRGECs treated with 4 mM Cd for 24 h. Positive staining of CFH was shown by orange-red fluorescence (n = 4). The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.
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    FIGURE 2. Cd increases <t>CFH</t> expression and secretion in HRGECs. (A, C, and E) ELISA of CFH secretion from HRGECs (A), human renal podocytes (HRPs) (C), and HRMCs (E) treated with 4 mM Cd (n = 4). (B, D, and F) mRNA expression of CFH gene in HRGECs (B), HRPs (D), and HRMCs (F) treated with 4 mM Cd (n = 4). Note: the scales are different in the y-axes. (G) Immunoblots of CFH from culture media of HRGECs. Coomassie Blue staining of the gel was presented as the loading control (n = 4). (H) Immunoblots of CFH from cell lysates of <t>HRGECs.</t> <t>GAPDH</t> was presented as the loading control (n = 4). (I) Immunoflourescence of CFH on cultured HRGECs treated with 4 mM Cd for 24 h. Positive staining of CFH was shown by orange-red fluorescence (n = 4). The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.
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    FIGURE 2. Cd increases CFH expression and secretion in HRGECs. (A, C, and E) ELISA of CFH secretion from HRGECs (A), human renal podocytes (HRPs) (C), and HRMCs (E) treated with 4 mM Cd (n = 4). (B, D, and F) mRNA expression of CFH gene in HRGECs (B), HRPs (D), and HRMCs (F) treated with 4 mM Cd (n = 4). Note: the scales are different in the y-axes. (G) Immunoblots of CFH from culture media of HRGECs. Coomassie Blue staining of the gel was presented as the loading control (n = 4). (H) Immunoblots of CFH from cell lysates of HRGECs. GAPDH was presented as the loading control (n = 4). (I) Immunoflourescence of CFH on cultured HRGECs treated with 4 mM Cd for 24 h. Positive staining of CFH was shown by orange-red fluorescence (n = 4). The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Cadmium Induces Glomerular Endothelial Cell-Specific Expression of Complement Factor H via the -1635 AP-1 Binding Site.

    doi: 10.4049/jimmunol.1800081

    Figure Lengend Snippet: FIGURE 2. Cd increases CFH expression and secretion in HRGECs. (A, C, and E) ELISA of CFH secretion from HRGECs (A), human renal podocytes (HRPs) (C), and HRMCs (E) treated with 4 mM Cd (n = 4). (B, D, and F) mRNA expression of CFH gene in HRGECs (B), HRPs (D), and HRMCs (F) treated with 4 mM Cd (n = 4). Note: the scales are different in the y-axes. (G) Immunoblots of CFH from culture media of HRGECs. Coomassie Blue staining of the gel was presented as the loading control (n = 4). (H) Immunoblots of CFH from cell lysates of HRGECs. GAPDH was presented as the loading control (n = 4). (I) Immunoflourescence of CFH on cultured HRGECs treated with 4 mM Cd for 24 h. Positive staining of CFH was shown by orange-red fluorescence (n = 4). The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.

    Article Snippet: After washing three times with TBST, the membranes were incubated with the secondary Ab (1:6000) at room temperature for 2 h. The primary Abs were rabbit anti-SAPK/JNK (9258), rabbit anti–phospho-SAPK/ JNK (4668), rabbit anti–c-Fos (2250), rabbit anti–c-Jun (9165), rabbit anti-GAPDH (2118) (Cell Signaling Technology), and a mouse mAb against human CFH (ab118820, OX24; Abcam, Cambridge, MA).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Staining, Control, Cell Culture

    FIGURE 4. Cd induces CFH expression through activation of the JNK pathway. (A) ELISA of CFH secretion from HRGECs pretreated with PD58059, SP600125, SB203580, PDTC, GSK690693 following 4 mM Cd treatment for 24 h (n = 6). (B) CFH mRNA expression from HRGECs pretreated with PD58059, SP600125, SB203580, PDTC, GSK690693 following 4 mM Cd treatment for 24 h (n = 6). (C) Immunoblots of JNK and phospho-JNK from HRGECs treated with 4 mM Cd (n = 4). (D) Immunoblots of c-Jun and c-Fos from HRGECs treated with 4 mM Cd (n = 4). GAPDH was presented as the loading control. The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Cadmium Induces Glomerular Endothelial Cell-Specific Expression of Complement Factor H via the -1635 AP-1 Binding Site.

    doi: 10.4049/jimmunol.1800081

    Figure Lengend Snippet: FIGURE 4. Cd induces CFH expression through activation of the JNK pathway. (A) ELISA of CFH secretion from HRGECs pretreated with PD58059, SP600125, SB203580, PDTC, GSK690693 following 4 mM Cd treatment for 24 h (n = 6). (B) CFH mRNA expression from HRGECs pretreated with PD58059, SP600125, SB203580, PDTC, GSK690693 following 4 mM Cd treatment for 24 h (n = 6). (C) Immunoblots of JNK and phospho-JNK from HRGECs treated with 4 mM Cd (n = 4). (D) Immunoblots of c-Jun and c-Fos from HRGECs treated with 4 mM Cd (n = 4). GAPDH was presented as the loading control. The mean of each column was compared to the mean of the control, and Student t test was used to determine the statistical significance. *p , 0.05, **p , 0.01.

    Article Snippet: After washing three times with TBST, the membranes were incubated with the secondary Ab (1:6000) at room temperature for 2 h. The primary Abs were rabbit anti-SAPK/JNK (9258), rabbit anti–phospho-SAPK/ JNK (4668), rabbit anti–c-Fos (2250), rabbit anti–c-Jun (9165), rabbit anti-GAPDH (2118) (Cell Signaling Technology), and a mouse mAb against human CFH (ab118820, OX24; Abcam, Cambridge, MA).

    Techniques: Expressing, Activation Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Control